Article
Mutational analysis of the Sar1 protein, a small GTPase which is essential for vesicular transport from the endoplasmic reticulum.
Journal of biochemistry - 1 Aug 1994
Nakano A, Otsuka H, Yamagishi M, Yamamoto E, Kimura K, Nishikawa S, Oka T
Abstract excerpt
SAR1 encodes a 21-kDa GTPase, which is required for vesicle formation from the endoplasmic reticulum in yeast. Although it belongs to the expanding small GTPase superfamily, there are interesting structural features that are unique to the Sar1 protein. We performed a site-directed mutational stud...
Topics
- Amino Acid Sequence
- Biological Transport, Active
- DNA Mutational Analysis
- Endoplasmic Reticulum
- Fungal Proteins
- GTP-Binding Proteins
- Molecular Sequence Data
- Monomeric GTP-Binding Proteins
- Phenotype
- Plasmids
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Transformation, Genetic
- Vesicular Transport Proteins