Article
In vivo analysis reveals that the interdomain region of the yeast proliferating cell nuclear antigen is important for DNA replication and DNA repair.
Genetics - 1 Oct 1996
Amin N S, Holm C
Abstract excerpt
To identify the regions of the proliferating cell nuclear antigen (PCNA) that are important for function in vivo, we used random mutagenesis to isolate 10 cold-sensitive (Cs-) and 31 methyl methanesulfonate-sensitive (Mmss) mutations of the PCNA gene (POL30) in Saccharomyces cerevisiae. Unlike th...
Topics
- Antigens, Fungal
- Cell Cycle Proteins
- Cold Temperature
- DNA Repair
- DNA Replication
- DNA, Fungal
- DNA, Single-Stranded
- Fungal Proteins
- G2 Phase
- Gene Expression Regulation, Fungal
- Genes, Lethal
- Mitosis
- Mutagenesis
- Phenotype
- Proliferating Cell Nuclear Antigen
- Replication Protein C
- S Phase
- Saccharomyces cerevisiae