Schizosaccharomyces pombe replication and repair proteins: proliferating cell nuclear antigen (PCNA).

doi:10.1006/meth.1999.0795

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Article

Schizosaccharomyces pombe replication and repair proteins: proliferating cell nuclear antigen (PCNA).

Methods (San Diego, Calif.) - 1 Jul 1999

Arroyo M P, Wang T S

Abstract excerpt

Schizosaccharomyces pombe has a cell cycle progression with distinctive phases that serves as a perfect model system for investigating DNA replication and repair of eukaryotic cells. Here, we use proliferating cell nuclear antigen (PCNA) of S. pombe to demonstrate how the function of this protein in both DNA replication and repair can be assessed by genetic and biochemical approaches. We describe a method of...

Topics

Cell Cycle
Cell Division
DNA Polymerase III
DNA Repair
DNA Replication
Fungal Proteins
Hydroxyurea
Methyl Methanesulfonate
Models, Molecular
Mutagenesis, Site-Directed
Mutagens
Phenotype
Proliferating Cell Nuclear Antigen
Protein Structure, Tertiary
Schizosaccharomyces
Temperature

Source

Open in PubMed

Identifiers

PMID: 10454995
DOI: 10.1006/meth.1999.0795

Genes

PCNA

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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