Article
The RAD52 recombinational repair pathway is essential in pol30 (PCNA) mutants that accumulate small single-stranded DNA fragments during DNA synthesis.
Genetics - 1 Feb 1998
Merrill B J, Holm C
Abstract excerpt
To identify in vivo pathways that compensate for impaired proliferating cell nuclear antigen (PCNA or Pol30p in yeast) activity, we performed a synthetic lethal screen with the yeast pol30-104 mutation. We identified nine mutations that display synthetic lethality with pol30-104; three mutations...
Topics
- Cell Division
- Cell Survival
- Centrifugation, Density Gradient
- DNA
- DNA Repair
- DNA Replication
- DNA-Binding Proteins
- Epistasis, Genetic
- Flow Cytometry
- Genes, pol
- Models, Molecular
- Mutagenesis
- Mutation
- Proliferating Cell Nuclear Antigen
- Rad52 DNA Repair and Recombination Protein
- Recombination, Genetic
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins