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Article

Stability of DNA repeats in Escherichia coli dam mutant strains indicates a Dam methylation-dependent DNA deletion process.

Gene - 27 Nov 2000

Troester H, Bub S, Hunziker A, Trendelenburg M F

Abstract excerpt

In this study we report on the stabilization of short direct repetitive DNA elements. We arranged a 20 bp SK-primer element in a direct repeat manner within the cloning vector pBluescript KS (+). This resulted in an array of 27 direct repeats consisting of 24 bp units. We show that this plasmid could only be propagated without deletion of repeats in dam mutant Escherichia coli hosts, whereas all efforts to use...

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Share to DNA Restriction Enzymes
Share to DNA Restriction-Modification Enzymes

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Rec A Recombinases

Share to Repetitive Sequences, Nucleic Acid

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Share to Sequence Homology, Nucleic Acid

Share to Site-Specific DNA-Methyltransferase (Adenine-Specific)

Share to Transformation, Bacterial

Source

Identifiers

PMID: 11111047
DOI: 10.1016/s0378-1119(00)00420-0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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