Article
Chimeric mice carrying 'regional' targeted deletion of the angiotensin type 1A receptor gene. Evidence against the role for local angiotensin in the in vivo feedback regulation of renin synthesis in juxtaglomerular cells.
The Journal of clinical investigation - 15 Oct 1996
Matsusaka T, Nishimura H, Utsunomiya H, Kakuchi J, Niimura F, Inagami T, Fogo A, Ichikawa I
Abstract excerpt
We have developed chimeric mice carrying 'regional' null mutation of the angiotensin type 1A (AT1A) receptor, the AT1 receptor subtype exclusively present in mouse juxtaglomerular (JG) cells. The chimeric mouse (Agtr1a -/- <--> +/+) is made up of wild-type (Agtr1a +/+) cells or cells homozygous for Agtr1a deletion (Agtr1a -/-). In the latter, the AT1A coding exon was replaced with a reporter gene, lacZ. In Agtr1a...
Topics
- Angiotensins
- Animals
- Chimera
- Feedback
- Gene Deletion
- Gene Targeting
- Juxtaglomerular Apparatus
- Lac Operon
- Mice
- Mice, Inbred C57BL
- Phenotype
- Receptors, Angiotensin
- Renal Artery