Article
Is there a negative TRE in the luciferase reporter cDNA?
Thyroid : official journal of the American Thyroid Association - 1 Aug 1996
Maia A L, Harney J W, Larsen P R
Abstract excerpt
Transient expression assays using a luciferase (LUC) reporter gene are often used in studies of positive and negative thyroid hormone response elements (TREs) or of wild-type and mutated thyroid hormone receptors (TRs). However, unliganded TR (the beta isoform > than the alpha) increases LUC expr...
Topics
- Animals
- Cell Line
- Genes, Reporter
- Humans
- Luciferases
- Mice
- Mutation
- Plasmids
- Rats
- Receptors, Thyroid Hormone
- Transfection
- Triiodothyronine