Article
Thyroid-hormone-dependent negative regulation of thyrotropin beta gene by thyroid hormone receptors: study with a new experimental system using CV1 cells.
The Biochemical journal - 1 Mar 2004
Nakano Keiko, Matsushita Akio, Sasaki Shigekazu, Misawa Hiroko, Nishiyama Kozo, Kashiwabara Yumiko, Nakamura Hirotoshi
Abstract excerpt
The molecular mechanism involved in the liganded thyroid hormone receptor suppression of the TSHbeta (thyroid-stimulating hormone beta, or thyrotropin beta) gene transcription is undetermined. One of the main reasons is the limitation of useful cell lines for the experiments. We have developed an assay system using non-pituitary CV1 cells and studied the negative regulation of the TSHbeta gene. In CV1 cells, the...
Topics
- Animals
- Cell Line
- Gene Silencing
- Genes, Reporter
- Haplorhini
- Humans
- Mutation
- Pituitary Gland
- Promoter Regions, Genetic
- Protein Isoforms
- Protein Structure, Tertiary
- Receptors, Cytoplasmic and Nuclear
- Receptors, Thyroid Hormone