Article
In vitro miscoding of alkylthymines with DNA and RNA polymerases.
Chemico-biological interactions - 1 Jan 2000
Saffhill R
Abstract excerpt
A series of poly(dT,RdT) polynucleotides containing O2-, 3- and O4-methyl- or ethyldeoxythymines have been prepared and used as templates for Pol I, Pol alpha and Pol R to investigate the miscoding properties of these modified bases with the different nucleotide polymerising enzymes. Only O4-alky...
Topics
- Alkylation
- Animals
- Chemical Phenomena
- Chemistry
- DNA
- DNA-Directed DNA Polymerase
- DNA-Directed RNA Polymerases
- Escherichia coli
- Mutagenicity Tests
- Mutation
- RNA
- Rats
- Spleen
- Thymine