Article
Use of shuttle vectors to study the molecular processing of defined carcinogen-induced DNA damage: mutagenicity of single O4-ethylthymine adducts in HeLa cells.
Nucleic acids research - 25 Jul 1990
Klein J C, Bleeker M J, Lutgerink J T, van Dijk W J, Brugghe H F, van den Elst H, van der Marel G A, van Boom J H, Westra J G, Berns A J
Abstract excerpt
We developed a simian virus 40 based shuttle vector system to study the molecular consequences of distinct carcinogen-induced DNA lesions in human cells. To establish the mutagenicity of O4-ethylthymine adducts, oligonucleotides carrying a single O4-ethylthymine adduct at a unique position were ligated into the vector molecules. Following replication in HeLa cells on average 23% of the progeny molecules carried a...
Topics
- Base Sequence
- Carcinogens
- Cell Line
- DNA Damage
- DNA Replication
- Genetic Vectors
- HeLa Cells
- Humans
- Kinetics
- Molecular Sequence Data