Article
FNR-DNA interactions at natural and semi-synthetic promoters.
Molecular microbiology - 1 Jan 1996
Green J, Irvine A S, Meng W, Guest J R
Abstract excerpt
Two rapid and convenient methods have been developed for the amplification and purification of FNR, the anaerobic transcription regulator of Escherichia coli. The overproduced proteins resemble wild-type FNR in their basic properties: oligomeric state, iron contents (up to 2.7 atoms per monomer),...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Binding Sites
- Chromatography, Gel
- DNA Footprinting
- DNA-Binding Proteins
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Escherichia coli Proteins
- Genes, Reporter
- Genotype
- Iron-Sulfur Proteins
- Lac Operon
- Molecular Sequence Data
- Nucleic Acid Conformation
- Promoter Regions, Genetic
- Protein Conformation