Article
Integration of three signals at the Escherichia coli nrf promoter: a role for Fis protein in catabolite repression.
Molecular microbiology - 1 Jul 2005
Browning Douglas F, Grainger David C, Beatty Christine M, Wolfe Alan J, Cole Jeffrey A, Busby Stephen J W
Abstract excerpt
Expression from the Escherichia coli nrf operon promoter is activated by the anaerobically triggered transcription factor, FNR, and by the nitrate/nitrite ion-controlled response regulators, NarL or NarP, but is repressed by the IHF and Fis proteins. Here, we present in vitro studies on the nrf promoter, using permanganate footprinting to measure open complex formation, and DNase I footprinting to monitor binding...
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