Article
The function of isolated domains and chimaeric proteins constructed from the transcriptional activators NifA and NtrC of Klebsiella pneumoniae.
Molecular microbiology - 1 Jan 1990
Drummond M H, Contreras A, Mitchenall L A
Abstract excerpt
A model for the domain structure of sigma 54-dependent transcriptional activators, based on sequence data, has been tested by examining the function of truncated and chimaeric proteins. Removal of the N-terminal domain of NtrC abolishes transcriptional activation, indicating that this domain is positively required for activator function. Over-expression of this domain as a separate peptide appears to titrate out...
Topics
- Bacterial Proteins
- Base Sequence
- DNA-Binding Proteins
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Klebsiella pneumoniae
- Molecular Sequence Data
- Mutation
- PII Nitrogen Regulatory Proteins
- Recombinant Fusion Proteins