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Article

Phenylalanine 30 plays an important role in receptor binding of verotoxin-1.

Molecular microbiology - 1 Feb 1996

Clark C, Bast D, Sharp A M, St Hilaire P M, Agha R, Stein P E, Toone E J, Read R J, Brunton J L

Abstract excerpt

The homopentameric B subunit of verotoxin 1 (VT1) binds to the glycosphingolipid receptor globotriaosylceramide (Gb3). We produced mutants with alanine substitutions for residues found near the cleft between adjacent subunits. Substitution of alanine for phenylalanine 30 (Phe-30) resulted in a fo...

Topics

Amino Acid Sequence
Bacterial Toxins
Calorimetry
Crystallography, X-Ray
Escherichia coli
Fourier Analysis
Glycolipids
Molecular Sequence Data
Mutation
Phenylalanine
Protein Binding
Protein Conformation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Receptors, Cell Surface

Shiga Toxin 1

Trihexosylceramides

Source

Identifiers

PMID: 8820657
DOI: 10.1046/j.1365-2958.1996.427962.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8820657-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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