Article
Application of N-terminally truncated DNA polymerase from Thermus thermophilus (delta Tth polymerase) to DNA sequencing and polymerase chain reactions: comparative study of delta Tth and wild-type Tth polymerases.
DNA research : an international journal for rapid publication of reports on genes and genomes - 30 Apr 1996
Arakawa T, Jongsareejit B, Tatsumi Y, Tanaka K, Ikeda K, Komatsubara H, Inoue H, Kawakami B, Oka M, Emi S, Yomo T, Shima Y, Negoro S, Urabe I
Abstract excerpt
N-Terminally truncated DNA polymerase from Thermus thermophilus (delta Tth polymerase) lacking 5'-3' exonuclease activity was used for DNA sequencing and polymerase chain reaction (PCR). In contrast to the high background of the sequencing ladder observed with the wild-type Tth polymerase, delta Tth polymerase gave readable sequencing patterns which extend up to more than 500 bases from the primer site on cycle...
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