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Article

Low fidelity mutants in the O-helix of Thermus aquaticus DNA polymerase I.

The Journal of biological chemistry - 25 Apr 1997

Suzuki M, Avicola A K, Hood L, Loeb L A

Abstract excerpt

We screened 67 mutants in the O-helix of Thermus aquaticus (Taq) DNA polymerase I (pol I) for altered fidelity of DNA synthesis. These mutants were obtained (Suzuki, M., Baskin, D., Hood, L., and Loeb, L. A. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 9670-9675) by substituting an oligonucleotide containing random sequences for codons 659-671, and selecting for complementation of a growth defect in Escherichia...

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Identifiers

PMID: 9111024
DOI: 10.1074/jbc.272.17.11228

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