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Article

Variants of a Thermus aquaticus DNA polymerase with increased selectivity for applications in allele- and methylation-specific amplification.

PloS one - 1 Jan 2014

Drum Matthias, Kranaster Ramon, Ewald Christina, Blasczyk Rainer, Marx Andreas

Abstract excerpt

The selectivity of DNA polymerases is crucial for many applications. For example, high discrimination between the extension of matched versus mismatched primer termini is desired for the detection of a single nucleotide variation at a particular locus within the genome. Here we describe the generation of thermostable mutants of the large fragment of Thermus aquaticus DNA polymerase (KlenTaq) with increased...

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Identifiers

PMID: 24800860
DOI: 10.1371/journal.pone.0096640
PMCID: PMC4011760

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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