Article
Selective expression of mutated p53 in human cells immortalized with either 4-nitroquinoline 1-oxide or 60Co gamma rays.
Cell structure and function - 1 Apr 1996
Mihara K, Iijima M, Kondo T, Namba M
Abstract excerpt
Many studies on in vitro transformation of human cells indicate that the cells must be immortalized before they can be neoplastically transformed, indicating that immortalization is a critical step in multistep neoplastic transformation of human cells. We immortalized three human cell lines by re...
Topics
- 4-Nitroquinoline-1-oxide
- Alleles
- Cell Line, Transformed
- Cobalt Radioisotopes
- DNA Mutational Analysis
- DNA, Neoplasm
- Fibroblasts
- Gamma Rays
- Gene Expression
- Humans
- Mutagens
- Mutation
- Polymerase Chain Reaction
- Precipitin Tests
- Retinoblastoma Protein
- Transformation, Genetic
- Tumor Suppressor Protein p53