Article
Yersinia enterocolitica aroA mutants as carriers of the B subunit of the Escherichia coli heat-labile enterotoxin to the murine immune system.
Microbial pathogenesis - 1 Aug 1990
O'Gaora P, Roberts M, Bowe F, Hormaeche C, Demarco de Hormaeche R, Cafferkey M, Tite J, Dougan G
Abstract excerpt
Plasmid p5F which directs the expression of the Escherichia coli heat-labile enterotoxin B subunit (LT-B) from the ptac promoter was introduced into the attenuated Yersinia enterocolitica O:8 aroA mutant strain YAM.1. YAM.1 (p5F) expressed high levels of cell-associated and secreted LT-B in a stable fashion when grown on normal laboratory medium. The strain was used as a live oral vaccine in BALB/c mice and...
Topics
- Animals
- Antibodies, Bacterial
- Antigens, Bacterial
- Bacterial Toxins
- Blotting, Western
- Enterotoxins
- Escherichia coli
- Escherichia coli Proteins
- Immunization
- Mice
- Mice, Inbred BALB C