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Article

Locating the unpaired cysteine of tissue-type plasminogen activator.

Protein engineering - 1 Mar 1996

Sehl L C, Nguyen H V, Berleau L T, Arcila P, Bennett W F, Keyt B A

Abstract excerpt

Variants of tissue-type plasminogen activator (t-PA) were constructed with selected cysteines replaced by alanine to evaluate the role of an unpaired cysteine, which has been presumed to be in the growth factor module. C75A, C83A, C84A and CC83-84AA variants of t-PA were expressed transiently in human embryonic kidney cells. The biochemical properties of these variants provided experimental evidence to identify...

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PMID: 8736495
DOI: 10.1093/protein/9.3.283

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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