Article
Signal and propeptide processing of human tissue plasminogen activator: activity of a pro-tPA derivative.
Biochemical and biophysical research communications - 30 Sept 1991
Berg D T, Grinnell B W
Abstract excerpt
We have explored the heterogeneity in the proteolytic processing of the N-terminus of human tissue plasminogen activator. We demonstrate that normal propeptide processing occurs following Arg-4, preceding the sequence Gly-Ala-Arg-Ser+1. Generation of the previously designated Ser+1 occurs via sec...
Topics
- Amino Acid Sequence
- Base Sequence
- Cell Line, Transformed
- Chromatography, Affinity
- Chromosome Deletion
- DNA
- Enzyme Precursors
- Genetic Variation
- Humans
- Kinetics
- Molecular Sequence Data
- Protein Processing, Post-Translational
- Protein Sorting Signals
- Recombinant Proteins
- Regulatory Sequences, Nucleic Acid
- Restriction Mapping
- Tissue Plasminogen Activator
- Transfection