Article
Site-directed mutagenesis in human tissue-plasminogen activator. Distinguishing sites in the amino-terminal region required for full fibrinolytic activity and rapid clearance from the circulation.
The Journal of biological chemistry - 5 Apr 1990
Ahern T J, Morris G E, Barone K M, Horgan P G, Timony G A, Angus L B, Henson K S, Stoudemire J B, Langer-Safer P R, Larsen G R
Abstract excerpt
Recombinant variants of tissue plasminogen activator (t-PA) containing either substitutions or deletions of amino acids within the fibronectin finger-like domain (residues 6-50) were found to exhibit widely varying in vivo clearance profiles in rats and fibrinolytic activity in 125I-fibrin clot l...
Topics
- Amino Acid Sequence
- Animals
- Cell Line
- Cricetinae
- Fibrinolysis
- Humans
- Male
- Metabolic Clearance Rate
- Molecular Sequence Data
- Mutation
- Plasminogen
- Protein Conformation
- Rats
- Rats, Inbred Strains