Article
High resolution analysis of functional determinants on human tissue-type plasminogen activator.
The Journal of biological chemistry - 15 Mar 1991
Bennett W F, Paoni N F, Keyt B A, Botstein D, Jones A J, Presta L, Wurm F M, Zoller M J
Abstract excerpt
Sixty-four variants of human tissue-type plasminogen activator (tPA) were produced using recombinant DNA techniques. Charged residues were converted to alanine in clusters of from one to four changes per variant; these clusters spanned all the domains of the molecule. The variants were expressed by mammalian cells and were analyzed for a variety of properties. Variants of tPA were found that had reduced activity...
Topics
- Amino Acid Sequence
- Binding Sites
- Fibrin
- Gene Expression Regulation
- Humans
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein Conformation
- Tissue Plasminogen Activator