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Article

High resolution analysis of functional determinants on human tissue-type plasminogen activator.

The Journal of biological chemistry - 15 Mar 1991

Bennett W F, Paoni N F, Keyt B A, Botstein D, Jones A J, Presta L, Wurm F M, Zoller M J

Abstract excerpt

Sixty-four variants of human tissue-type plasminogen activator (tPA) were produced using recombinant DNA techniques. Charged residues were converted to alanine in clusters of from one to four changes per variant; these clusters spanned all the domains of the molecule. The variants were expressed by mammalian cells and were analyzed for a variety of properties. Variants of tPA were found that had reduced activity...

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PMID: 1900516

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