Article
In vitro and in vivo phenotypes resulting from deletion of the high temperature requirement A (htrA) gene from the bovine vaccine strain Brucella abortus S19.
Veterinary microbiology - 1 Apr 1996
Robertson G T, Elzer P H, Roop R M
Abstract excerpt
An htrA deletion mutant was created in the bovine vaccine strain, B. abortus S19, by replacing the majority of the htrA gene with a kanamycin resistance gene. Antibiotic selection for a double crossover event yielded kanamycin-resistant, ampicillin-sensitive colonies confirmed by Southern and wes...
Topics
- Animals
- Brucella Vaccine
- Brucella abortus
- Brucellosis, Bovine
- Cattle
- Female
- Gene Deletion
- Genes, Bacterial
- Hydrogen Peroxide
- Hypersensitivity, Delayed
- Mice
- Mice, Inbred BALB C
- Microbial Sensitivity Tests
- Phenotype
- Puromycin
- Time Factors