Article
Characterization and genetic complementation of a Brucella abortus high-temperature-requirement A (htrA) deletion mutant.
Infection and immunity - 1 Oct 1994
Elzer P H, Phillips R W, Kovach M E, Peterson K M, Roop R M
Abstract excerpt
In order to evaluate the biological function of the Brucella abortus high-temperature-requirement A (HtrA) stress response protein homolog, the majority of the htrA gene was deleted from the chromosome of B. abortus 2308 via gene replacement. In contrast to the parental strain, the resulting htrA deletion mutant, designated PHE1, failed to grow on solid medium at 40 degrees C and demonstrated increased...
Topics
- Animals
- Brucella abortus
- Female
- Gene Deletion
- Genetic Complementation Test
- Heat-Shock Proteins
- Hydrogen Peroxide
- Mice
- Mice, Inbred BALB C
- Mutation
- Periplasmic Proteins