Article
Cloning, characterization and construction of htrA and htrA-like mutants of Brucella abortus and their survival in BALB/c mice.
Microbial pathogenesis - 1 Jul 1994
Tatum F M, Cheville N F, Morfitt D
Abstract excerpt
A genomic library of Brucella abortus S2308 was screened for expression of recombinant proteins recognized by sera from mice and from cattle infected with B. abortus. A positive clone, BA1, expressing a 50 kDa peptide was recognized by both sera. Plasmid pBA1, isolated from BA1, was shown by rest...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Bacterial
- Bacterial Proteins
- Base Sequence
- Blotting, Southern
- Blotting, Western
- Brucella abortus
- Brucellosis
- Cloning, Molecular
- Genomic Library
- Heat-Shock Proteins
- Male
- Mice
- Mice, Inbred BALB C
- Microbial Sensitivity Tests
- Molecular Sequence Data
- Mutation