Article
Sensitive detection of loss of heterozygosity in the TP53 gene in pancreatic adenocarcinoma by fluorescence-based single-strand conformation polymorphism analysis using blunt-end DNA fragments.
Genes, chromosomes & cancer - 1 Mar 1996
Sugano K, Nakashima Y, Yamaguchi K, Fukayama N, Maekawa M, Ohkura H, Kakizoe T, Sekiya T
Abstract excerpt
We have developed a fluorescence-based single-strand conformation polymorphism analysis to detect Haelil-sensitive polymorphic sites in intron 1 of the TP53 gene. It is important to treat the PCR products with Klenow fragment to remove a 3'-protruding nucleotide from the amplified DNA fragments added during the reaction in order to obtain a single peak for each allele. A comparison of the signal profiles of two...
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