Article
Semiautomated quantitative detection of loss of heterozygosity in the tumor suppressor gene p53.
BioTechniques - 1 Jun 1995
Hahn M, Matzen S E, Serth J, Pingoud A
Abstract excerpt
The most frequently altered gene in diverse tumor types is the tumor suppressor gene p53. Typically, normal function is inactivated by point mutation of one allele and deletion of the other. Therefore, loss of heterozygosity (LOH) of intragenic polymorphic markers is a strong indication for p53 involvement in a cancerous lesion. This study shows that a highly polymorphic short tandem repeat (STR) within intron 1...
Topics
- Alleles
- Base Sequence
- DNA
- DNA Primers
- Fluorescent Dyes
- Gels
- Gene Deletion
- Genes, p53
- Heterozygote
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction