Sensitive detection of p53 gene mutations by a 'mutant enriched' PCR-SSCP technique.

doi:10.1093/nar/26.5.1356

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Article

Sensitive detection of p53 gene mutations by a 'mutant enriched' PCR-SSCP technique.

Nucleic acids research - 1 Mar 1998

Behn M, Schuermann M

Abstract excerpt

For the rapid and sensitive detection of p53 'hot spot' mutations, we combined polymerase chain reaction based single-strand conformational polymorphism (PCR-SSCP) analysis with sequence specific-clamping by peptide nucleic acids (PNAs) in a one-step reaction tube protocol. For this purpose, we d...

Topics

Alleles
Base Sequence
DNA Primers
DNA, Neoplasm
Exons
Genes, p53
Humans
Lung Neoplasms
Mutation
Nucleic Acids
Peptides
Point Mutation
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Sensitivity and Specificity

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