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Article

Sensitive detection of p53 gene mutations by a 'mutant enriched' PCR-SSCP technique.

Nucleic acids research - 1 Mar 1998

Behn M, Schuermann M

Abstract excerpt

For the rapid and sensitive detection of p53 'hot spot' mutations, we combined polymerase chain reaction based single-strand conformational polymorphism (PCR-SSCP) analysis with sequence specific-clamping by peptide nucleic acids (PNAs) in a one-step reaction tube protocol. For this purpose, we designed two PNA molecules comprising aa 246-250 of exon 7 and aa 270-275 of exon 8, respectively, to suppress the...

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Identifiers

PMID: 9469850
DOI: 10.1093/nar/26.5.1356
PMCID: PMC147406

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