Article
Polymerase chain reaction amplification specificity: incidence of allele dropout using different DNA preparation methods for heterozygous single cells.
Journal of assisted reproduction and genetics - 1 Feb 1996
Gitlin S A, Lanzendorf S E, Gibbons W E
Abstract excerpt
PURPOSE: The purpose was to evaluate methods of DNA preparation in a single cell to determine the ability to amplify and correctly diagnose a targeted gene. METHODS: One- or two-cell lymphoblasts (n = 100/group), heterozygous for the normal and 4-base pair insertion on exon 11 of the beta-hexosam...
Topics
- Alleles
- Artifacts
- Cell Fractionation
- Cells, Cultured
- DNA
- DNA Mutational Analysis
- Dithiothreitol
- Exons
- Feasibility Studies
- Freezing
- Heterozygote
- Hot Temperature
- Humans
- Hydroxides
- Isoenzymes
- Lymphocytes
- Microchemistry
- Nitrogen