Article
[Low volume amplification in single cell separation and inspection].
Fa yi xue za zhi - 1 Apr 2012
Han Jun-Ping, Li Cai-Xia, Yan Hong, Zhu Dian, Li Gen-Ping, Hu Lan
Abstract excerpt
OBJECTIVE: To establish optimal amplification conditions with the application of 0.2 mL test tube in single cell separation and inspection. METHODS: Oral epithelium cell suspension was prepared. Five or ten cells were collected with 0.2 mL test tube. Then DNA were amplified with Identifiler Plus kit in three different conditions in which the proteinase K addition, gold enzyme concentration in PCR reaction, and...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
