Article
Molecular comparison of single cell MDA products derived from different cell types.
Reproductive biomedicine online - 1 Jul 2009
Glentis Stavros, SenGupta Sioban, Thornhill Alan, Wang Rubin, Craft Ian, Harper Joyce Catherine
Abstract excerpt
The quality of DNA obtained from single cells for molecular analysis is primarily dependent on cell type and cell lysis. Multiple displacement amplification (MDA) amplifies the DNA isothermally with the use of Phi29 polymerase and random hexamer primers. The efficiency and accuracy of MDA was assessed on different cell types (buccal cells, lymphocytes, fibroblasts) using two multiplex PCR reactions that have been...
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