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Article

Allele drop-out can occur in alleles differing by a single nucleotide and is not alleviated by preamplification or minor template increments.

Genetic testing - 1 Jan 1998

Hahn S, Garvin A M, Di Naro E, Holzgreve W

Abstract excerpt

The ability to analyze the genetic material of single cells by the PCR opens up new prospects for diagnostics. Because only two copies of the genetic template are available for amplification, a problem that frequently arises when examining heterozygous loci in single cells is allele drop-out (ADO). ADO results from the preferential amplification of one of a pair of heterozygous alleles, in which the other allele...

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Source

Identifiers

PMID: 10464616
DOI: 10.1089/gte.1998.2.351

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ADO

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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