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Article

Preferential PCR amplification of alleles: mechanisms and solutions.

PCR methods and applications - 1 May 1992

Walsh P S, Erlich H A, Higuchi R

Abstract excerpt

The preferential PCR amplification of one allele relative to another in a heterozygous sample could result in an incorrect or ambiguous genetic typing of that sample. There are several mechanisms that could potentially lead to such preferential PCR amplification. First, preferential amplification can result from significant GC% differences between alleles if the conditions of the reaction (denaturation...

Topics

Alleles
Base Sequence
Genes, MHC Class II
Genetic Markers
Genotype
HLA-DQ Antigens
HLA-DQ alpha-Chains
Humans
Molecular Sequence Data
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid

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Stochastic Processes

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Identifiers

PMID: 1477658
DOI: 10.1101/gr.1.4.241

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1477658-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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