Article
A technique for eliminating allele specific amplification failure during DNA amplification of heterozygous cells for preimplantation diagnosis.
Molecular human reproduction - 1 Nov 1997
El-Hashemite N, Delhanty J D
Abstract excerpt
Advances in techniques of molecular biology have made possible the amplification of specific genes from single cells. This has a major clinical application in preimplantation diagnosis of monogenic disorders. However, the incidence of allele specific amplification failure (allele drop out) in heterozygous single cells can lead to misdiagnosis and the transfer of affected embryos. Few studies have been done to...
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