Allele dropout in sequential PCR and FISH analysis of single cells (cell recycling).

doi:10.1007/BF02072532

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Allele dropout in sequential PCR and FISH analysis of single cells (cell recycling).

Journal of assisted reproduction and genetics - 1 Feb 1996

Rechitsky S, Freidine M, Verlinsky Y, Strom C M

Abstract excerpt

PURPOSE: Our purpose was to investigate the feasability of using sequential PCR and FISH analysis of single cells for preimplantation diagnosis. METHODS: Protocols for sequential PCR and FISH analysis of a single fibroblast (cell recycling) were optimized for six loci and the rates of allele spec...

Topics

Alleles
Amelogenin
Anemia, Sickle Cell
Artifacts
Base Sequence
Cells, Cultured
Cystic Fibrosis
Cystic Fibrosis Transmembrane Conductance Regulator
DNA
DNA Mutational Analysis
DNA Primers
Dental Enamel Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Evaluation Studies as Topic

Feasibility Studies

Female

Fibroblasts

Genetic Markers

Globins

Source

Open in PubMed

Identifiers

PMID: 8688583
DOI: 10.1007/BF02072532

Genes

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8688583-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Allele dropout in sequential PCR and FISH analysis of single cells (cell recycling).PMID 8688583Read ↗

Multiple genetic diagnoses from single cells using multiplex PCR: reliability and allele dropout.Semantic proximity Read ↗

Strategies for preimplantation genetic diagnosis of single gene disorders by DNA amplificationSemantic proximity Read ↗

Single cell polymerase chain reaction for preimplantation genetic diagnosis: methods, strategies, and limitations.Semantic proximity Read ↗

Allele dropout in polar bodies and blastomeres.Semantic proximity Read ↗

Increasing the denaturation temperature during the first cycles of amplification reduces allele dropout from single cells for preimplantation genetic diagnosis.Semantic proximity Read ↗

Detailed investigation of factors influencing amplification efficiency and allele drop-out in single cell PCR: implications for preimplantation genetic diagnosis.Semantic proximity Read ↗

Recycling of a single human blastomere fixed on a microscopic slide for sexing and diagnosis of specific mutations by various types of polymerase chain reaction.Semantic proximity Read ↗

Allelic drop-out and preferential amplification in single cells and human blastomeres: implications for preimplantation diagnosis of sex and cystic fibrosis.Semantic proximity Read ↗

A technique for eliminating allele specific amplification failure during DNA amplification of heterozygous cells for preimplantation diagnosis.Semantic proximity Read ↗

A comparison of different lysis buffers to assess allele dropout from single cells for preimplantation genetic diagnosis.Semantic proximity Read ↗

Reduced allele dropout in single-cell analysis for preimplantation genetic diagnosis of cystic fibrosis.Semantic proximity Read ↗

Fluorescent PCR: a new technique for PGD of sex and single-gene defects.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.