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Article

Allele dropout in sequential PCR and FISH analysis of single cells (cell recycling).

Journal of assisted reproduction and genetics - 1 Feb 1996

Rechitsky S, Freidine M, Verlinsky Y, Strom C M

Abstract excerpt

PURPOSE: Our purpose was to investigate the feasability of using sequential PCR and FISH analysis of single cells for preimplantation diagnosis. METHODS: Protocols for sequential PCR and FISH analysis of a single fibroblast (cell recycling) were optimized for six loci and the rates of allele specific dropout (ADO) were determined. RESULTS: Conditions that allow reliable genotyping of single cells in lysis buffer...

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PMID: 8688583
DOI: 10.1007/BF02072532

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ADO

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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