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Article

A comparison of different lysis buffers to assess allele dropout from single cells for preimplantation genetic diagnosis.

Prenatal diagnosis - 1 Jun 2001

Thornhill A R, McGrath J A, Eady R A, Braude P R, Handyside A H

Abstract excerpt

Single cell polymerase chain reaction (PCR) for preimplantation genetic diagnosis (PGD) requires high efficiency and accuracy. Allele dropout (ADO), the random amplification failure of one of the two parental alleles, remains the most significant problem in PCR-based PGD testing since it can result in serious misdiagnosis for compound heterozygous or autosomal dominant conditions. A number of different strategies...

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Source

Identifiers

PMID: 11438956
DOI: 10.1002/pd.109

Genes

PGD
ADO
ALB
CFTR
LAMA3
PKP1
SDS

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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