Increasing the denaturation temperature during the first cycles of amplification reduces allele dropout from single cells for preimplantation genetic diagnosis.

Abstract excerpt

Single cell polymerase chain reaction (PCR) for preimplantation genetic diagnosis (PGD) needs to be highly efficient and accurate. In some single cells from human embryos presumed to be heterozygous for the delta F508 deletion causing cystic fibrosis (CF), we recently observed random amplification failure of one of the two parental alleles following nested PCR. To investigate allele dropout (ADO), we have...

Topics

• Alleles
• Cystic Fibrosis
• DNA Primers
• Embryonic Development
• Female
• Fertilization in Vitro
• Globins
• Heterozygote
• Humans
• Lymphocytes
• Mutation
• Polymerase Chain Reaction