Article
Two-hybrid interaction of a human UBC9 homolog with centromere proteins of Saccharomyces cerevisiae.
Molecular & general genetics : MGG - 23 May 1996
Jiang W, Koltin Y
Abstract excerpt
Using a two-hybrid system, we cloned a human cDNA encoding a ubiquitin-conjugating enzyme (UBC), hUBC9, which interacts specifically with all three subunits of the Saccharomyces cerevisiae centromere DNA-binding core complex, CBF3. The hUBC9 protein shows highest homology to a new member of the UBC family: 54% identity to S. cerevisiae Ubc9p and 64% identity to Schizosaccharomyces pombe (Sp) hus5. Overexpression...
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