Article
Thrombin receptor activating mutations. Alteration of an extracellular agonist recognition domain causes constitutive signaling.
The Journal of biological chemistry - 12 Jan 1996
Nanevicz T, Wang L, Chen M, Ishii M, Coughlin S R
Abstract excerpt
Constitutively active thrombin receptors were generated while constructing chimeric receptors to identify the structural basis for thrombin receptor agonist specificity. Substitution of eight amino acids from the Xenopus receptor's second extracellular loop (XECL2B) for the cognate sequence in th...
Topics
- Amino Acid Sequence
- Animals
- Calcium
- GTP-Binding Proteins
- Humans
- Molecular Sequence Data
- Mutation
- Receptors, Thrombin
- Recombinant Fusion Proteins
- Sequence Alignment
- Sequence Analysis
- Signal Transduction