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Article

Essential role for the second extracellular loop in C5a receptor activation.

Nature structural & molecular biology - 1 Apr 2005

Klco Jeffery M, Wiegand Christina B, Narzinski Kirk, Baranski Thomas J

Abstract excerpt

More than 90% of G protein-coupled receptors (GPCRs) contain a disulfide bridge that tethers the second extracellular loop (EC2) to the third transmembrane helix. To determine the importance of EC2 and its disulfide bridge in receptor activation, we subjected this region of the complement factor 5a receptor (C5aR) to random saturation mutagenesis and screened for functional receptors in yeast. The cysteine...

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PMID: 15768031
DOI: 10.1038/nsmb913

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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