Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Random mutagenesis of the human adenosine A2B receptor followed by growth selection in yeast. Identification of constitutively active and gain of function mutations.

Molecular pharmacology - 1 Mar 2004

Beukers Margot W, van Oppenraaij Joris, van der Hoorn Patrick P W, Blad Clara C, den Dulk Hans, Brouwer Jaap, IJzerman Adriaan P

Abstract excerpt

To gain insight in spontaneous as well as agonist-induced activation of the human adenosine A2B receptor, we applied a random mutagenesis approach in yeast to create a large number of receptor mutants and selected mutants of interest with a robust screening assay based on growth. The amino acid sequence of 14 mutated receptors was determined. All these mutated receptors displayed constitutive activity. In...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acid Sequence
Share to CHO Cells

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Cricetinae

Share to Cyclic AMP

Share to Molecular Sequence Data

Share to Mutagenesis

Share to Mutation

Share to Saccharomyces cerevisiae

Source

Identifiers

PMID: 14978249
DOI: 10.1124/mol.65.3.702

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Random mutagenesis of the human adenosine A2B receptor followed by growth selection in yeast. Identification of constitutively active and gain of function mutations.PMID 14978249Read ↗

Characterization of cancer-related somatic mutations in the adenosine A2B receptor.Semantic proximity Read ↗

Domains for activation and inactivation in G protein-coupled receptors--a mutational analysis of constitutive activity of the adenosine A2B receptor.Semantic proximity Read ↗

Scanning mutagenesis in a yeast system delineates the role of the NPxxY(x)(5,6)F motif and helix 8 of the adenosine A(2B) receptor in G protein coupling.Semantic proximity Read ↗

Loss of constitutive activity is correlated with increased thermostability of the human adenosine A2A receptor.Semantic proximity Read ↗

Cancer-Related Somatic Mutations in Transmembrane Helices Alter Adenosine A1 Receptor Pharmacology.Semantic proximity Read ↗

Cancer-related somatic mutations alter adenosine A1 receptor pharmacology-A focus on mutations in the loops and C-terminus.Semantic proximity Read ↗

ZM241385, DPCPX, MRS1706 are inverse agonists with different relative intrinsic efficacies on constitutively active mutants of the human adenosine A2B receptor.Semantic proximity Read ↗

Systematic identification of mutations that constitutively activate the angiotensin II type 1A receptor by screening a randomly mutated cDNA library with an original pharmacological bioassay.Semantic proximity Read ↗

Ligand rescue of constitutively active mutant receptors.Semantic proximity Read ↗

Constitutively active G protein-coupled receptor mutants: implications on receptor function and drug action.Semantic proximity Read ↗

Engineering a human P2X2 receptor with altered ligand selectivity in yeast.Semantic proximity Read ↗

Cancer-Associated Mutations of the Adenosine A2A Receptor Have Diverse Influences on Ligand Binding and Receptor Functions.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.