Article
Systematic identification of mutations that constitutively activate the angiotensin II type 1A receptor by screening a randomly mutated cDNA library with an original pharmacological bioassay.
Proceedings of the National Academy of Sciences of the United States of America - 20 Jun 2000
Parnot C, Bardin S, Miserey-Lenkei S, Guedin D, Corvol P, Clauser E
Abstract excerpt
The constitutive activation of G-protein-coupled receptors is a major new approach to investigating their physiopathology and pharmacology. A large number of spontaneous and site-directed mutations resulting in constitutive activity have been identified, but systematic mapping of the amino acids involved for a given receptor would be extremely useful for complete elucidation of the molecular mechanisms underlying...
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- Animals
- Biological Assay
- CHO Cells
- Cricetinae
- Gene Library
- Humans
- Molecular Sequence Data
- Mutation
- Receptors, Angiotensin
- Signal Transduction