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Article

Characterization of the XRCC1-DNA ligase III complex in vitro and its absence from mutant hamster cells.

Nucleic acids research - 11 Dec 1995

Caldecott K W, Tucker J D, Stanker L H, Thompson L H

Abstract excerpt

The human DNA repair protein XRCC1 was overexpressed as a histidine-tagged polypeptide (denoted XRCC1-His) in Escherichia coli and purified in milligram quantities by affinity chromatography. XRCC1-His complemented the mutant Chinese hamster ovary cell line EM9 when constitutively expressed from a plasmid or when introduced by electroporation. XRCC1-His directly interacted with human DNA ligase III in vitro to...

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Identifiers

PMID: 8532526
DOI: 10.1093/nar/23.23.4836
PMCID: PMC307472

Genes

XRCC1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Research trail→

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Characterization of the XRCC1-DNA ligase III complex in vitro and its absence from mutant hamster cells.PMID 8532526Read ↗

An interaction between the mammalian DNA repair protein XRCC1 and DNA ligase III.Semantic proximity Read ↗

Construction of human XRCC1 minigenes that fully correct the CHO DNA repair mutant EM9.Semantic proximity Read ↗

Altered DNA ligase III activity in the CHO EM9 mutant.Semantic proximity Read ↗

Mitochondrial DNA ligase III function is independent of Xrcc1.Semantic proximity Read ↗

Molecular cloning of the human XRCC1 gene, which corrects defective DNA strand break repair and sister chromatid exchange.Semantic proximity Read ↗

Functional capacity of XRCC1 protein variants identified in DNA repair-deficient Chinese hamster ovary cell lines and the human populationSemantic proximity Read ↗

Partial correction of the single-strand break repair defect in the CHO mutant EM9 by electroporated recombinant XRCC1 protein.Semantic proximity Read ↗

Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells.Semantic proximity Read ↗

DNA damage levels and biochemical repair capacities associated with XRCC1 deficiency.Semantic proximity Read ↗

A single amino acid substitution in DNA-PKcs explains the novel phenotype of the CHO mutant, XR-C2.Semantic proximity Read ↗

Characterization of the hamster FancG/Xrcc9 gene and mutations in CHO UV40 and NM3.Semantic proximity Read ↗

DNA-ligase activities appear normal in the CHO mutant EM9.Semantic proximity Read ↗

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