Article
Characterization of the XRCC1-DNA ligase III complex in vitro and its absence from mutant hamster cells.
Nucleic acids research - 11 Dec 1995
Caldecott K W, Tucker J D, Stanker L H, Thompson L H
Abstract excerpt
The human DNA repair protein XRCC1 was overexpressed as a histidine-tagged polypeptide (denoted XRCC1-His) in Escherichia coli and purified in milligram quantities by affinity chromatography. XRCC1-His complemented the mutant Chinese hamster ovary cell line EM9 when constitutively expressed from...
Topics
- Animals
- Antibodies, Monoclonal
- CHO Cells
- Cricetinae
- DNA Ligase ATP
- DNA Ligases
- DNA Repair
- DNA-Binding Proteins
- Escherichia coli
- HeLa Cells
- Histidine
- Humans
- Mutation
- Poly-ADP-Ribose Binding Proteins
- Recombinant Proteins
- X-ray Repair Cross Complementing Protein 1
- Xenopus Proteins