Article
Targeted disruption of H2B-V encoding a particular H2B histone variant causes changes in protein patterns on two-dimensional polyacrylamide gel electrophoresis in the DT40 chicken B cell line.
The Journal of biological chemistry - 22 Dec 1995
Takami Y, Takeda S, Nakayama T
Abstract excerpt
The chicken H2B gene family comprises eight members (H2B-I to H2B-VIII), which are all located in two major histone gene clusters. All of them have been shown to encode four different protein variants (classes I to IV). In the DT40 chicken B cell line, the H2B-V gene, encoding the class III H2B v...
Topics
- Animals
- B-Lymphocytes
- Cell Line
- Chickens
- Electrophoresis, Gel, Two-Dimensional
- Electrophoresis, Polyacrylamide Gel
- Gene Expression
- Genetic Variation
- Histones
- Multigene Family
- RNA, Messenger
- Recombinant Proteins
- Restriction Mapping
- Transfection