Article
Application of denaturing gradient gel blots to detect p53 mutations in X-ray-transformed mouse C3H 10T1/2 clones.
Molecular carcinogenesis - 1 Jan 1993
Krolewski B, Little J B
Abstract excerpt
The denaturing-gradient gel electrophoresis (DGGE) blot technique is a highly sensitive method developed to detect sequence differences (e.g., single base changes and small deletions) in genomic DNA. We used this method to screen for mutations within the p53 locus of independent X-ray-transformed...
Topics
- Animals
- Cell Line
- Cell Transformation, Neoplastic
- Electrophoresis, Agar Gel
- Exons
- Genes, p53
- In Vitro Techniques
- Mice
- Mutation
- Nucleic Acid Denaturation
- Polymorphism, Genetic
- X-Rays