Kinetics of bisulfite-induced cytosine deamination in single-stranded DNA.

doi:10.1021/bi00065a003

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Article

Kinetics of bisulfite-induced cytosine deamination in single-stranded DNA.

Biochemistry - 13 Apr 1993

Chen H, Shaw B R

Abstract excerpt

The rate of bisulfite-induced deamination of cytosine to uracil in single-stranded (ss) DNA at physiological temperature and pH was monitored by a sensitive genetic assay. The assay is based on reversion of a mutation in the lacZ alpha gene of bacteriophage M13mp2 and employs ung- (NR9404) and un...

Topics

Bacteriophages
Codon
Cytosine
DNA Glycosylases
DNA Repair
DNA, Single-Stranded
Deamination
Escherichia coli
Kinetics
Mutation
N-Glycosyl Hydrolases
Sulfites
Transformation, Bacterial
Uracil
Uracil-DNA Glycosidase
beta-Galactosidase

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