Article
Deamination of cytosine-containing pyrimidine photodimers in UV-irradiated DNA. Significance for UV light mutagenesis.
The Journal of biological chemistry - 13 Oct 1995
Barak Y, Cohen-Fix O, Livneh Z
Abstract excerpt
The realization that cytosine in cyclobutyl pyrimidine dimers rapidly deaminates to uracil raised the possibility that this chemical transformation, rather than an enzymatic polymerase error, is the major mutagenic step in UV mutagenesis. We have established a sensitive bioassay system that enabl...
Topics
- Base Composition
- Cytosine
- DNA Damage
- Deamination
- Dose-Response Relationship, Radiation
- Escherichia coli
- Genotype
- Hydrogen-Ion Concentration
- Kinetics
- Mutagenesis
- Plasmids
- Pyrimidine Dimers
- Temperature
- Ultraviolet Rays