Differential expression of alkaline phosphatase in clones of human osteoblast-like cells.

doi:10.1002/jbmr.5650080306

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Article

Differential expression of alkaline phosphatase in clones of human osteoblast-like cells.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research - 1 Mar 1993

Manduca P, Sanguineti C, Pistone M, Boccignone E, Sanguineti F, Santolini F, Federici A

Abstract excerpt

We established cultures of cells growing out from adult bone chips and maintained them through 12 passages in culture. The cultures showed osteoblastic phenotype accompanied by synthesis of collagen type I, osteonectin, alkaline phosphatase, and osteocalcin. We report the characterization of 21 c...

Topics

Adult
Alkaline Phosphatase
Bone and Bones
Clone Cells
Collagen
Electrophoresis, Polyacrylamide Gel
Female
Humans
Male
Middle Aged
Molecular Weight
Osteoblasts
Osteonectin
Phenotype
Precipitin Tests

Source

Open in PubMed

Identifiers

PMID: 8456585
DOI: 10.1002/jbmr.5650080306

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8456585-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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