Article
Subcloning of three osteoblastic cell lines with distinct differentiation phenotypes from the mouse osteoblastic cell line KS-4.
Bone - 1 Nov 1996
Yamashita T, Ishii H, Shimoda K, Sampath T K, Katagiri T, Wada M, Osawa T, Suda T
Abstract excerpt
Three distinct osteoblastic cell lines (KS418, KS460, and KS483) were subcloned from the mouse osteoblastic KS-4 cells, which possessed the abilities not only to differentiate into mature osteoblasts, but also to support osteoclast differentiation in coculture with spleen cells. The order of the...
Topics
- Actins
- Alkaline Phosphatase
- Animals
- Bone Morphogenetic Protein 7
- Bone Morphogenetic Proteins
- Calcification, Physiologic
- Calcitriol
- Cell Differentiation
- Cell Line
- Cells, Cultured
- Cloning, Molecular
- Collagen
- Culture Media
- DNA, Complementary
- Glycerophosphates
- Mice
- Microscopy, Electron
- Osteoblasts
